ترجمه آماده – اسپکتروفوتومتری مشتق شده UV-VIS در شیمی تحلیلی

The paper deals with theoretical and instrumental aspects connected with derivative spectrophotometry. Ideal absorption spectra and their derivatives from 1st to 6th order are consequently discussed. The influence of intensity and width of peaks and relative position of the bands of the analyzed substances on the quality of the derivative spectra obtained has been discussed (choice of derivative order and measurement technique). Next, the dependence of the apparatus parameters on the course of derivative curves is described. Methods of obtaining derivatives with particular consideration of the Savitzky-Golay numeric differentiation technique are presented. The dependence of the number of spectrum points and degree of polynomial fit on the smoothness and differentiation of spectra is described. Possibilities of improving the selectivity and sensitivity of determination using derivative spectrophotometry are presented. Examples of applying this technique for the determination of inorganic and organic analytes are shown.